Excel sheet containing raw data of Figs.

Fig 1C including positive cells of tyrosine hydroxylase+ cell and JEV-E+ cell at MOCK, 3dpi, 6dpi and 9dpi. Fig 1D including mean fluorescence intensity of tyrosine hydroxylase+ cell and JEV-E+ cell at MOCK, 3dpi, 6dpi and 9dpi. Fig 2B including positive cells of NLRP3+ cell and ASC+ cell at MOCK, 3dpi, 6dpi and 9dpi. Fig 2D including positive cells of caspase-1+cell with tyrosine hydroxylase+ cell at MOCK, 3dpi, 6dpi and 9dpi; mean fluorescence intensity of caspase-1+cell with tyrosine hydroxylase+ cell at MOCK, 3dpi, 6dpi and 9dpi; GSDMD+ cell with tyrosine hydroxylase+ cell at MOCK, 3dpi, 6dpi and 9dpi; mean fluorescence intensity of GSDMD+ cell with tyrosine hydroxylase+ cell at mock, 3dpi, 6dpi and 9dpi; Fig 2E including Pearson coefficients of JEV+/TH+, TH+/caspase-1+, TH+/GSDMD+ and JEV+/IBA-1+ cell at MOCK, 3dpi, 6dpi and 9dpi. Fig 3A including qPCR of mice about NLRP3, ASC, caspase-1, GSDMD, IL-1β, IL-18, TNF-α and HMGB1 at MOCK, 3dpi, 6dpi and 9dpi. Fig 3C including Western blot of mice about NLRP3, ASC, pro-caspase-1, caspase-1, GSDMD-FL, GSDMD-N, pro-IL-1β and IL-1β at MOCK, 3dpi, 6dpi and 9dpi. Fig 4B including extracellular LDH of dopaminergic neurons (MN9D cell) at MOCK, 12hpi, 24hpi, 36hpi, 48hpi and 60hpi. Fig 4C including JEV copies of dopaminergic neurons (MN9D cell) at MOCK, 12hpi, 24hpi, 36hpi, 48hpi and 60hpi. Fig 5A including qPCR of dopaminergic neurons (MN9D cell) about NLRP3, ASC, caspase-1, GSDMD, IL-1β, IL-18 and TNF-α at MOCK, 12hpi, 24hpi, 36hpi, 48hpi and 60hpi. Fig 5C including Western blot of dopaminergic neurons (MN9D cell) about NLRP3, ASC, pro-caspase-1, caspase-1, GSDMD-FL, GSDMD-N, pro-IL-1β and IL-1β at MOCK, 12hpi, 24hpi, 36hpi, 48hpi and 60hpi. Fig 7B including NESS score of mice in group DMEM, group MCC950, group JEV and group JEV + MCC950 from day1 to day 10. Fig 7E including qPCR of mice about NLRP3, ASC, caspase-1, GSDMD, IL-1β, IL-18, TNF-α and HMGB1 at MOCK, MCC950, 3dpi of JEV, 3dpi of JEV + MCC950, 6dpi of JEV, 6dpi of JEV + MCC950, 9dpi of JEV and 9dpi of JEV + MCC950. Fig 7G including Western blot of mice about NLRP3, ASC, pro-caspase-1, caspase-1, GSDMD-FL, caspase-1, pro-IL-1β and IL-1β at MOCK, MCC950, 3dpi of JEV, 3dpi of JEV + MCC950, 6dpi of JEV, 6dpi of JEV + MCC950, 9dpi of JEV and 9dpi of JEV + MCC950. Fig 8B including positive cells of IBA-1+ cell and JEV-E+ cell at MOCK, 3dpi, 6dpi and 9dpi; mean fluorescence intensity of IBA-1++ cell and JEV-E+ cell at MOCK, 3dpi, 6dpi and 9dpi. Fig 8D including qPCR of microglial (BV2 cell) about NLRP3, ASC, caspase-1, GSDMD, IL-1β, IL-18, TNF-α and HMGB1 at MOCK, 12hpi, 24hpi, 36hpi, 48hpi and 60hpi. Fig 8F including Western blot of microglial (BV2 cell) about NLRP3, ASC, pro-caspase-1, caspase-1, GSDMD-FL and GSDMD-N at MOCK, 12hpi, 24hpi, 36hpi and 48hpi. (XLSX)