Characterization of endothelial and smooth muscle cells from different canine vessels

Canine primary endothelial cells (ECs) and vascular smooth muscle cells (vSMCs) were isolated from the vena cava, vena porta and aorta. All tissue sources were derived from three donors for accurate comparison and to reduce inter-animal variation. We used a recently established technique for the isolation of primary ECs with a lesser chance of contamination that can also be used on small vessels. This same vessel was also used for the isolation of vSMCs whole transriptome data of canine endothelial cells and vascular smooth muscle cells produced on Illumina NextSeq500 using a single-end 75 bp configuration