The primers used to amplify wild type and mutant PPARG alleles.
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Notes: The underlined sequences indicate the enzyme cutting sites EcoRI (GAATTC) and KpnI (GGTACC), which were artificially added into the forward and reverse primers, respectively. The sequences in bold show the translation start site (ATG) and translation stop site (CTA, which is the reverse complement of the TAG). The sequences in frame show the Asp7Gly mutation (the codon was changed from GAT to GGT).
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2015-12-02
