Gene expression of cervical cells and immortalized keratinocytes transfected with whole genomes of HPV16 and HPV18

Human ectocervical and endocervical cells immortalized by HPV16 E6/E7 (designated as Ect and End, respectively) and normal human immortalized keratinocytes (NIKS), were transfected with whole genomes of HPV16 or HPV18, and were subsequently grown in standard monolayer culture until passage 2. Total RNAs were extracted and subjected to TORAY 3D-gene analysis service using the 3D-Gene Human Oligo chip 25K (TORAY, Tokyo, Japan). Ect, End and NIKS cells with or without HPV16/18 genome (total nine cell samples) were analized.