High-throughput sequencing analysis of miRNAs expression profiles in the thoracic ascending aortic aneurysm (TAA) tissues and plasma samples.

In the present study, we evaluated miRNA expression profiles of 32 patient aortic aneurysm tissue and plasma samples using Illumina next-generation sequencing platform. A total of 20 miRNA were differentially expressed in tissues, 17 miRNAs - in plasma samples. Differentially expressed miRNAs determined in the present study could be applied for thoracic ascending aneurysm diagnosis. For the tissue analysis, 8 TAA samples and 6 healthy aortic control samples were collected. The 3 of 7 TAA plasma samples were isolated from the same patients, which were included in the TAA tissue group. Moreover, 4 plasma samples from the same TAA patients 3 months after the surgery were investigated. A control group of 7 plasma samples was isolated from unrelated healthy patients. All samples were analyzed using Illumina MiSeq v2 miRNA sequencing by Illumina MiSeq system.