MALDI Imaging Mass Spectrometry of a Mouse Kidney
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https://figshare.com/articles/dataset/MALDI_Imaging_Mass_Spectrometry_of_a_Mouse_Kidney/735961/1
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Instrument: Autoflex III Smartbeam (Bruker, Bremen, Germany) Linear mode (m/z 2000 - 20000) Negative ion mode Matrix: 2,4,6-Trihydroxyacetophenone Spatial resolution: 150 µm <br> Tissue - Mouse Kidney (BI/6, C57BL/6JRccHsd) - Healthy - Snap frozen in liquid N2 immediately after harvest - 12 µm section (on Leica cryostat @ - 30°C) Matrix application Sublimation @ 160°C for 12 min 30 sec Recrystallization With 1 mL of 5% MeOH, 5% Acetic Acid @ 80°C for 2 min 30 sec (starting from RT) Tissue wash According to Yang and Caprioli, <em>Anal. Chem.</em> <strong>2011</strong>, 83, 5728-5734 ; Rinse protocol D
提供机构：
figshare
创建时间：
2016-01-18
