The Illumina MiSeq platform was used to investigate and identify the microbial community based on 16S rRNA V4 region.. Microbial community composition in manure biogas plants

Biogas production from anaerobic digestion is an important renewable energy source and optimization and upgrading of biogas is aided by gaining knowledge of the microbial organisms involved in biogas production. Nine Danish biogas reactors was investigated by amplicon sequencing. The study aimed to optimize DNA extraction from manure biogas plants. The influence of eDNA and free DNA from lysed cells were investigated by introducing propidium monoazide treatment.