Adaptation to chronic ER stress enforces pancreatic beta-cell plasticity

The next-generation-sequencing (NGS) of ribosome footprintings and poly(A) enriched RNA-seq have been proformed to examine transcriptional and translational changes in an mouse insulinoma cell MIN6 under acute and chronic endoplasmic reticular stresses. Mouse insulinoma cell MIN6 treated with 200uM of Cyclopiazonic acid for 1 (CPA1) and 18 hours (CPA18). An 18 hour DMSO treatment served as the control (CPA0). Ribo-seq and poly(A) enriched RNA-seq (mRNA-seq) have been proformed. Three independent biological replications were proformed.