Equilibrium binding constants and Hill coefficients for the association of nuclease domains with synthetic or E. coli lipid LUVs.

Incubations were performed in 10 mM Kpi, pH 7.5, at 25°C, with a lipid concentration of 200 µM. Standard errors are derived from the non-linear regression analysis. N.A. indicates absence of cooperativity. Nuclease titrations were carried out at least twice, with similar results.