PJ34- and ABT888-induced reduction of PARs influences histone H3 and H4 acetylation.

(A) Representative Western blot of whole cell extracts from cells treated with the inhibitor PJ34 (5μM) at the indicated times relative to untreated cells (NT, value ~1), run on a 15% SDS-PAGE, and probed with anti-acetyl-histone H3 and anti-C-terminal of H3 antibodies to measure the relative level of H3 acetylation. Error bars indicate the standard deviation of data obtained from three independent experiments. (B) Same as in (A) but hybridisation was performed with anti-acetyl-histone H4 and anti-C-terminal of H4 antibodies to measure the relative level of H4 acetylation. (C) The same extracts used in panels A and B were run on an 8% SDS-PAGE and probed with anti-PAR antibody to visualize ADP-ribose polymers level. (D) Same as in (A) but from cells treated with the inhibitor ABT888 (0.5 μM). (E) Same as in (D) but hybridisation was performed with anti-acetyl-histone H4 and anti-C-terminal of H4 antibodies to measure the relative level of H4 acetylation. (F) The same extracts used in panels D and E were run on an 8% SDS-PAGE and probed with anti-PAR antibody to visualize PARs level. *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001