Biosynthesis of membrane-spanning lipids in Thermoanaerobacter ethanolicus

This study was conducted to identify the genes involved in the synthesis of membrane-spanning lipids in Thermoanaerobacter ethanolicus JW 200. Thermoanaerobacter ethanolicus was cultivated under anaerobic conditions in 80 ml batch cultures in 250 ml serum bottles. Cultures were incubated either at 60°C or 45°C growth temperatures. Samples were derived from three replicates at each condition. The cells were harvested at mid exponential, late exponential, and stationary phases.