Simultaneous Detection of Gene Expression of insects, nematode and bacterial specific genes to monitor the infection.

In this study, NanoString technology gene expression quantification platform was used to study the expression of toxin genes causing infections from Bacteria (Photorhabdus and Xenorhabdus), Nematode (H.indica, S.riobrave , S.carpocapsae) specific genes for detection and Immune related genes from the infected insects (Spodoptera frugiperda and Galleria mellonella). The study revealed the expression of different immune related genes from the infected insects (Spodoptera frugiperda and Galleria mellonella) and helped in understanding the trend of expression of gene in the samples from the healthy condition to the death stage. Variations in gene expression were seen as per the expectation. The worms were infected with bacteria from genus of Photorhabdus and Xenorhabdus. The experiment included samples in three stages, Uninfected, Infected and Dead. Uninfected samples were taken as control. Sufficient amount of RNA was extracted suitable for NanoString custom assay and after subjecting to quantity and quality check it was proceeded for NanoString custom multiplexed assay. Total samples- 10.