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Tests on the PCR amplification efficiency of primer pairs.

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Figshare2015-12-02 更新2026-04-29 收录
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https://figshare.com/articles/dataset/Molecular_Cloning_and_Characterization_of_Juvenile_Hormone_Acid_Methyltransferase_in_the_Honey_Bee_Apis_mellifera_and_Its_Differential_Expression_during_Caste_Differentiation/742364
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Serial 8× dilutions of cDNA samples were used as templates to generate relative standard curves. The regression equation, determination coefficient (r2) and PCR amplification efficiency (E) were calculated and presented. A) test on the primer pair for the target gene AmJHAMT; B) test on the primer pair for the reference gene actin. (PDF)
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2015-12-02
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