Cloning, characterization, antibacterial activity and expression of hamp in pond loach (Misgurnus anguillicaudatus) after bacterial challenge with Aeromonas hydrophila

Supplementary Fig. 1 The detailed primer reports of hamp 1a and 1b which were used to determine the expression pattern in qRT-PCR
Supplementary Fig. 2 Phylogenic tree of hepcidin amino acid sequences constructed using the neighbor-joining method.
Supplementary Fig. 3 The plot shows the posterior probabilities of inside/outside/transmembrane helices.
Supplementary Fig. 4 (A) Three-dimensional structures of M. anguillicaudatus Hepcidin protein obtained using SWISS-MODEL
Supplementary Table 1. GenBank accession numbers for hepcidin homologs
Supplementary Table 2. Assessment of predicted three-dimensional structure of hepcidin protein by different validation methods.
Supplementary Table 3. Predicted protein interactions for the M. anguillicaudatus hepcidin 1 inferred using STRING algorithm
Supplementary Table 4. Basal mRNA expression of the two paralogs in different tissues relative to β-actin
Supplementary Table 5. mRNA expression of the two paralogs in different tissues after the challenge with A. hydrophila