Table_4_Genome-wide survey and expression analysis of Dof transcription factor family in sweetpotato shed light on their promising functions in stress tolerance.xlsx

DNA-binding with one finger (Dof) transcription factors play a crucial role in plant abiotic stress regulatory networks, although massive Dofs have been systematically characterized in plants, they have not been identified in the hexaploid crop sweetpotato. Herein, 43 IbDof genes were detected to be disproportionally dispersed across 14 of the 15 chromosomes of sweetpotato, and segmental duplications were discovered to be the major driving force for the expansion of IbDofs. The collinearity analysis of IbDofs with their related orthologs from eight plants revealed the potential evolutionary history of Dof gene family. Phylogenetic analysis displayed that IbDof proteins were assigned into nine subfamilies, and the regularity of gene structures and conserved motifs was consistent with the subgroup classification. Additionally, five chosen IbDof genes were shown to be substantially and variably induced under various abiotic conditions (salt, drought, heat, and cold), as well as hormone treatments (ABA and SA), according to their transcriptome data and qRT-PCR experiments. Consistently, the promoters of IbDofs contained a number of cis-acting elements associated with hormone and stress responses. Besides, it was noted that IbDof2 had transactivation activity in yeasts, while IbDof-11/-16/-36 did not, and protein interaction network analysis and yeast two-hybrid experiments revealed a complicated interaction connection amongst IbDofs. Collectively, these data lay a foundation for further functional explorations of IbDof genes, especially with regards to the possible application of multiple IbDof members in breeding the tolerant plants.

DNA结合型单指转录因子（Dof）在植物非生物胁迫调节网络中扮演着至关重要的角色。尽管大量Dof因子已在植物中系统性地被表征，但在六倍体作物甘薯中尚未发现。本研究中，检测到43个IbDof基因在甘薯的15条染色体中的14条上不均匀分布，并发现片段重复是IbDof家族扩张的主要驱动因素。通过将IbDof基因与来自八种植物的相应直系同源基因进行同线性分析，揭示了Dof基因家族的潜在进化历史。系统发育分析表明，IbDof蛋白被归为九个亚家族，基因结构和保守基序的规律性与亚家族分类一致。此外，根据其转录组数据和qRT-PCR实验，五个选择的IbDof基因在盐、干旱、高温和低温等非生物条件下，以及激素处理（ABA和SA）下显示出显著且可变的诱导。一致地，IbDof基因的启动子中包含与激素和胁迫反应相关的多个顺式作用元件。此外，值得注意的是，IbDof2在酵母中具有转活化活性，而IbDof-11/-16/-36则不具有，蛋白质相互作用网络分析和酵母双杂交实验揭示了IbDof之间的复杂相互作用关系。总之，这些数据为IbDof基因的进一步功能研究奠定了基础，尤其是关于多个IbDof成员在培育耐胁迫植物中可能应用的探索。