ChIP
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https://figshare.com/articles/dataset/ChIP/6484646
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To
verify the direct activation of miR-92b from SP1, we performed ChIP assays with
PCI-4A cells to respectively affirm the binding reaction of the 5 binding sites
in miR-92b promoter region. The
binding of each predicted sites were evaluated with 7 parallel groups: (A)
experiment group: immunoprecipitated with SP1 antibody and amplified with
targeted primers; (B) RT-Input1: without immunoprecipitation and amplified with
targeted primers; (C) negative control group: immunoprecipitated with host IgG
and amplified with targeted primers; (D) PCR negative control: using ddH<sub>2</sub>O
as template and amplified with targeted primers; (E) positive control group:
immunoprecipitated with RNA polymerase II and amplified with GAPDH primers; (F)
RT-Input2: without immunoprecipitation and amplified with GAPDH primers; (G)
WB-Input: western bloting SP1 protein expression in total DNA-protein
crosslink.The results indicated that there was a significant
enrichment of SP1 protein at the first 3 binding sites, whereas no obvious
reaction was found in site 4 and site 5.
提供机构：
figshare
创建时间：
2019-02-08
