BPGv2: HiFi and Hi-C raw data

Pangenomes are collections of annotated genome sequences of multiple individuals of a species. The structural variants uncovered by these datasets are a major asset to genetic analysis in crop plants. Here, we report a pangenome of barley comprising long-read sequence assemblies of 76 wild and domesticated genomes. We selected (i) diverse domesticated germplasm with a focus on genebank accessions from barley's center of diversity in the Middle East; (ii) 23 accessions of barley's conspecific wild progenitor H. vulgare subsp. spontaneum from across that taxon's geographic range; and (iii) cultivars of agronomic or scientific relevance. Examples of the last category are Bonus, Foma and Bowman, three parents of classical mutants. Genome sequences of each accession were assembled to contig-level from PacBio HiFi accurate long reads and scaffolded with conformation capture sequencing (Hi-C) data to chromosome-scale pseudomolecules.