Gene expression profiling of Acute Myeloid Leukemia

The transcriptional profile of acute myeloid leukemia (AML) cells changes according to the disease molecular and genomic properties and to the microenvironmental features. Moreover, it shapes the interaction with the tissue and immune microenvironment. We analyzed the gene expression profile of 61 AML cases (Affymetrix Human Transcriptome Array 2.0, Thermo Fisher Scientific) in order to identify investigate the potential involvement of adrenomedullin in AML and the alterations having a putative causal and/or tolerogenic role towards aneuploidy. The gene expression profile of 61 AML cases was determined using Affymetrix Human Transcriptome Array 2.0, in order to identify alterations with a putative causal and/or tolerogenic role towards aneuploidy. Bone marow cells from AML patients (more than or equal to 80% blast cells) were used for RNA extraction and hybridization on microarrays.