Identification of seminal proteins related to the inhibition of mate searching in female crickets

In response to the reduction in fitness associated with sperm competition, males are expected to evolve tactics that hinder female remating. For example, females often display a post-mating reduction in their sexual receptivity that has been shown to be mediated by proteins contained in a male’s seminal fluid (sfps). However, although there has been comprehensive research on sfps in genetically well characterized species, few non-model species have been studied in such detail. We initially confirm that female Australian field crickets, Teleogryllus oceanicus, do display a significant reduction in their mate searching behavior 24 hours after mating. This effect was still apparent three days after mating but was entirely absent after one week. We then attempted to identify the sfps that might play a role in inducing this behavioral response. We identified two proteins, ToSfp022 and ToSfp011, that were associated with the alteration in female post-mating behavior. The knockdown of both pro...