October 2018 18S and COI data

Mapping file and raw data associated with October 2018 18S and COI MiSeq sequencing runs. Water samples were collected above and below culverts on Mt. Desert Island (sample site coordinates provided). Culvert samples are named by overpass (C1-4 and M1-3, see coordinate file) and location (above or below = A or B). Additional water samples were collected from rockpools along the shore of the Schoodic Peninsula. Soil samples were collected adjacent to Northeast Temperate Network plots (sample site coordinates provided, sample names correspond to NTEN site). All samples were frozen prior to DNA extraction. Water sampling, DNA extraction, and 18S laboratory processing followed the methods described in Paulson and Martin (2021): https://doi.org/10.1007/s00442-019-04526-6. Soil samples (230 cm³ each) were individually homogenized, then DNA was extracted with Qiagen DNeasy PowerSoil kits. Downstream laboratory processing followed the methods described for the water samples. All PCR reactions were performed in triplicate for the 18S amplicons, and duplicate for the COI amplicons. COI info: gene targeting and sequence processing followed the methods described by Madden et al. (2016 - https://doi.org/10.1111/mec.13900), with the exception of OTU clustering at 97% rather than 99%. BOLD data were used for taxonomy assignment. Samples that did not produce amplicon were excluded from sequencing.18S info: sequence processing followed the pipeline described at https://github.com/leffj/data-tutorials/blob/master/amplicon_data_processing_tutorial/amplicon_data_processing-16S.md, modified for 18S data. The SILVA 127 database was used for taxonomy assignment.Data in OTU tables are unrarified.