Self-organization of human dorsal-ventral forebrain structures by light induced SHH

We report the use of a light stimulus to geometrically confine SHH expression in neuralizing hESCs. This led to the self-organization of mediolateral neural patterns. scRNA-seq analysis established that these structures represent the dorsal-ventral forebrain, at the end of the first month of development. Here, we show that morphogen light-stimulation is a scalable tool that induces self-organizing centers. Overall design: scRNA-seq using 10X genomics platform of light induced SHH structures in hESC during differentiation. Two replica at day 14.