Quantitative real-time PCR detection for B. cockerelli endogenous BC-ATPase mRNAs after feeding on the plants infected with virus expressing BC-ATPase interfering sequence.

1The plants used for virus infection and B. cockerelli feeding.2The virus vector expressing BC-ATPase and control GFP sequences for plant inoculation.3The number of psyllids used for qRT-PCR analysis in one experiment. For each experiment, the same numbers of GFP samples were used as controls for test sample.4Teneral adult B. cockerelli or nymphs for feeding experiments and RNA abundance detection were indicated. All of the samples labeled “Nymph” were done using leaf disc method, and the samples labeled “Adult” were done using whole plant feeding experiment.5The mRNA abundance of BC-ATPase gene after feeding on virus-ATPase –infected plants was shown as test sample, and the average value of the control GFP group was designated as 1. Expression of BC-ATPase was normalized to the level of BC-Actin in the same sample.6Differences between GFP and test group were calculated using the Bonferroni t-test. Single asterisk indicates p