CRISPR screen of radiation resistant genes in NSCLC

Radiotherapy is an important treatment for non-small cell lung cancer (NSCLC). It not only kills tumor cells directly, but also promotes the efficacy of immunotherapy. However, resistance to radiotherapy is still an unavoidable clinical challenge. In this study, we used radiation-sensitive H460 cell line to stably express Cas9 and CRISPR GeCKO v2 library (A and B). Then a single 4GY irradiation was given, and after the cells resumed proliferation, the total genome was extracted and second-generation sequencing was performed. Genes directly associated with resistance to radiotherapy were identified in comparison to controls without any treatment. Overall design: The Cas9 and GeCKO v2 library expressing H460 celsl were separated into Control and IR groups. Each group has three repeats. The IR group was treated with 4 Gy ionizing radiation. Then, the total genome of the 6 samples was extracted and sequenced.