Transcriptional profiling of PNPase KO liver hepatocytes. Transcriptional profiling of PNPase KO liver hepatocytes

Transcriptional profiling of PNPase KO liver hepatocytes (HepKO) generated from Albumin-Cre/wt (liver-specific) Pnpt1 fl/fl C57BL/6J mice. Samples comparing liver hepatocyte PNPase KO (HepKO) cells to litter-, age-, and sex-matched wildtype hepatocyte control cells. The goal of this experiment was to determine effects of PNPase loss on the total RNA transcriptome under physiologic in vivo conditions. Overall design: Two conditions: PNPASE KO liver hepatocytes (HepKO) vs matched WT (control) hepatocytes. Biological replicates: Two biological replicates in each condition; one age-, litter-, and sex- matched male pair of HepKO and WT mice, and another age-, litter-, and sex- matched female pair of HepKO and WT mice. Comparison was performed in sex-matched fluor-reversed pairs.