Investigating effect of TMEM184B mutation on hippocampal gene expression in 5 month and 15 month old mice

The objective of this study was to determine whether TMEM184B influenced the expression of genes important for neuronal function or memory. We isolated hippocampus tissue from 3-4 mice of wild type or TMEM184B gene trap mutant mice at 5 months and 15 months of age. We then isolated total RNA and performed paired-end bulk RNA sequencing本研究旨在探究TMEM184B是否会影响神经元功能或记忆相关基因的表达。我们分别于5月龄和15月龄时，从3-4只野生型（wild type）或TMEM184B基因陷阱突变（gene trap mutant）小鼠中分离海马组织，随后提取总RNA并开展双端批量RNA测序（paired-end bulk RNA sequencing）。