Whole genome sequences of S.cerevisiae clones from Q and NQ cells enriched lines. Saccharomyces cerevisiae S288C

Adaptive roles of SSY1 and SIR3 during cycles of growth and starvation in Saccharomyces cerevisiae populations enriched for quiescent (Q) or non-quiescent (NQ) cellsTo investigate the genes that may be involved in the differentiation of Q and NQ cells we serially propagated yeast populations that were enriched for either only Q or only NQ cell types over many repeated growth-starvation cycles. After 30 cycles (equivalent to 300 generations), each enriched population produced a higher proportion of the enriched cell type compared to the starting population, suggestive of adaptive change. Whole genome sequencing of clones from Q and NQ enriched lines revealed mutations in genes involved in nutrient stress response and survival in limiting nutrients (ECM21, RSP5, MSN1, SIR4, IRA2) in both Q and NQ lines, but also differences between the two lines: NQ line clones had recurrent independent mutations affecting the SPS amino acid sensing pathway, while Q line clones had recurrent, independent mutations in SIR3 and FAS1.