Identification of constitutively-expressed immune effectors in the house fly (Musca domestica) and the transcription factors that regulate them

In this study we fed groups of female house flies one of two Gram-negative bacteria (Pseudomonas aeruginosa and Escherichia coli) or control broth and used RNA-seq to determine differential gene expression at 4 hours post-feeding. Flies that ingested P. aeruginosa induced expression of genes encoding digestive proteins in the gut but did not induce AMPs. Flies in all treatment groups constitutively expressed some paralogs of lysozyme and AMP genes at the transcript level. RNA-seq performed on flies infected with either Pseudomonas aeruginosa, Escherichia coli, or control medai 4 hours after infection