Comparison of insulin release and cellular insulin content before and after glucose-induced replication.

Insulin released into culture medium from day 0 to 3 and from day 12 to 15 and also in extracts cells collected on day 3 and day 15 was measured. Data was expressed as function of the number of cells counted on these days. Data represent means ± SEM; *, p<0.05; **, p<0.01 versus corresponding day 3 values; n = 4.