Proteolysis of zyxin by HtrA1 induces nuclear translocation of the resulting C-terminal fragment of zyxin

We identified two neo-N termini derived from proteolytic cleavage of the mechanotransducer zyxin at Asp149 and Val276. We correlated these proteolytic events with the activity of caspase-1 and serine protease HtrA1, respectively, and observed nuclear translocation of the fragment zyxin277-572. To evaluate the biological role of nuclear zyxin we used Tandem Mass Tags (TMT)-based quantitative proteomics to compare protein abundances in HeLa cell ectopically expressing zyxin277-572, full length zyxin and control cells.