Comparison of transcripts detected in intergenic regions in regards to previous analysis.

The numbers and names of non overlapping transcripts found in intergenic regions are given for each study and compared to the present study. The locus of each transcript has been considered. Three studies [26], [28], [46] used in silico prediction in first intention and confirmed the existence of transcripts by experimental validation such as Northern blot or Race-PCR. Only the sRNAs which have been experimentally tested have been taken into account. Otherwise, our study as those of Roberts [45], Anderson [44] and Abu-Qatouseh [47] are based on direct experimental approaches as Affymetrix microarrays, or cloning/sequencing strategy. The number of detected transcripts varies according to the methodologies and the strain used by the author. The study of Geissmann [26] also predicted the known cis-acting elements such as the metabolite-sensing riboswitches, the T-box motifs and several protein-mediated antiterminaison events.