RNA sequencing of mouse epidermis and papillomas treated with 20% camphor white oil or acetone vehicle

Camphor white oil (CWO) application was found to induce regression of papillomas in a mouse chemical carcinogenesis model. In healthy epidermis, CWO induced increased proliferation of basal keratinocytes. RNA-sequencing was performed on epidermal cells 24 hours after CWO or vehicle application (N=3 mice/treatment), and on 4 pooled papillomas harvested after 6 weeks of treatment (N=3 mice/treatment). Sequencing identified transcripts that may alter skin physiology and induce tumor regression. Overall design: Examination of 2 tissue types treated with camphor white oil or vehicle.