Expression data from distal colorectal tissues of Pdgfc+/+ and Pdgfc-/- mice with or without irradiation

Radiation therapy for malignancies in the pelvic region leads to a crucial complication called radiation proctopathy(RP) with acute changes in bowel habits or long-term morbidity. We used microarrays to detail the genome-wide profiles of mice with different genotypes (Pdgfc+/+ and Pdgfc-/- ) under irradiation treatment or not. The distal colorectal tissues of Pdgfc+/+ and Pdgfc-/- mice with or without irradiation were selected for RNA extractionon, amplification, label, hybridization and scan on Affymetrix microarrays, and finally calculated by MAS 5.0 algorithm, Affy packages in R to get the genome-wide profiles of different groups.