Expression data from transdetermined Drosophila eye antennal imaginal disc by winged eye overexpression.

Drosophila imaginal disc cells exhibit a remarkable ability to switch cell fates under various perturbations, a phenomenon known as transdetermination (TD). The winged eye (wge) gene induces eye-to-wing TD by its overexpression in eye imaginal discs using eye specific Gal4 driver (eyeless-Gal4). Gene network controlling this process, however, is largely unclear. Additionally, we identified that heterochromatin-related histone methyltransferase Su(var)3-9 is essential for wge-mediated TD. We used microarray to detail the global gene network underlying wge-mediated eye-to-wing TD, and the involvement of Su(var)3-9 in the gene network. Third instar larval eye imaginal discs were dissected for RNA extraction and hybridization on Affymetrix microarray. Flies from each genotype were used (I: eyeless-Gal4, II: UAS-wge, III: ey-Gal4; UAS-wge (ey > wge), IV: ey-Gal4 UAS-wge; Su(var)3-91/Su(var)3-92). Three biologic replicates were obtained for each.