MIR200B/C microRNAs bind NOTCH1 mRNA

Translation of NOTCH1 mRNA is inhibited by microRNAs miR-200B and miR-200C, which bind to the 3'UTR of NOTCH1 mRNA. Levels of miR-200B and miR-200C are decreased in pancreatic cancer cells with an EMT (epithelial to mesenchymal transition) phenotype, and the EMT phenotype is reversed by exogenous overexpression of miR-200B/C microRNAs, suggesting that miR-200B and mir-200C may be acting as tumor suppressors.

NOTCH1 信使RNA的翻译受microRNA miR-200B和miR-200C的抑制，它们与NOTCH1信使RNA的3'非编码区（3'UTR）结合。在具有上皮间质转化（EMT）表型的胰腺癌细胞中，miR-200B和miR-200C的水平降低，而外源性过表达miR-200B/C microRNA可通过逆转EMT表型，提示miR-200B和miR-200C可能作为肿瘤抑制因子发挥作用。