Overexpression of IGF-1 During Early Development Expands the Number of Mammary Stem Cells and Primes them for Transformation

In this paper we report transcriptomic profiles of mammary stem cells (MaSCs) from female BK5.IGF1 transgenic and age-matched WT mice, generated by single-cell RNA-sequencing technology. We found that IGF-1 stimulated quiescent MaSCs to enter the cell cycle and increased their expression of genes involved in proliferation, plasiticity, tumorigenesis, invasion, and metastasis. Overall design: Examination of transcriptomic profiles in MaSCs from BK5.IGF-1 and WT female mice.