Transcriptomic analysis of REVOLUTA and KANADI cell-types of Arabidopsis shoot apical meristem

Dorso-ventral (DV) patterning at the Shoot Apical meristem (SAM) is essential for organogenesis in Arabidopsis. Dorsal and ventral gene expressed domains are separated by boundaries from where the new organ initiation starts. Here by using cell-types specific transcriptomics approach, we have identified dorsal, ventral and boundary specific genes in SAM. 5 different cell-types, and 4 biological replicates for each cell-type were collcted through FACS, and used for RNA-Seq analysis: BFP: Epidermal boundary BFPGFP: Ventral Epidermal cell-types BFPYFP:  Dorsal Epidermal cell-types GFP:  Ventral Sub-epidermal cell-types YFP: Dorsal Sub-epidermal cell-types