Subcommissural organ (SCO) contributes to brain development via SCO-secreted peptides.. Subcommissural organ (SCO) contributes to brain development via SCO-secreted peptides.

To explore the mechanism of SCO-derived peptides, we performed RNAseq in cultured neurons added with SCO peptide and control neurons which added with DPBS to identify the expression of some unique genes and possible pathway related to the function of SCO derived peptides. We cofirmed the role of SCO derived peptide which contribute in neuronal development,especially projection developent and cell survival. development and neuronal survival . Overall design: Differential expression analysis and Gene Set Enrichment Analysis of RNA seq data. We performed differential expression analysis and Gene Set Enrichment Analysis of RNA seq data obtained from cultured neurons added with SCO-derived peptides and cultured neurons added with DPBS