Integrin-alpha 4 (itga4) scRNA-seq

This project contains 15 samples processed using Parse platform for scRNA-seq, falling under the following four conditions:<i>integrin-alpha 4</i> (<i>itga4</i>) mutant young adult, 6mpf - 4 samples (2M, 2F)<i>vascular cell adhesion molecule 1b</i> (<i>vcam1b</i>) mutant young adult, 6mpf - 3 samples (2M, 1F)WT young adult, 6mpf - 4 samples (2M, 2F)WT old adult, 24mpf - 4 samples (2M, 2F)Whole kidney marrow (WKM) was isolated from each sample and red blood cell lysis was performed before cell counts, fixation, and processing for sequencing (according to Parse platform protocols). Sample barcoding, library prep, and sequencing performed by UW-Madison Gene Expression Center and Biotech Center. Initial processing, alignment, and QC of raw sequencing reads performed by UW-Madison Bioinformatics Resource Center.Raw reads can be found in the "DGE_unfiltered" folder for each individual sample, or for full experiment groups under the "all-sample"/"all-wells" files. The "DGE_filtered" files in the mentioned locations contain the pre-processed and aligned reads, as well as the count matrices (reads were aligned to custom GRCz11 genome file provided with data). <br>