Gene expression analysis of a panel of human fibroblasts that generate an aligned (anisotropic) vs non-aligned extracellular matrix

A panel of human fibroblasts derived from patient samples (vulval, oral, cervical and breast carcinoma and metastatic melanoma) were seperated according to their ability to generate an aligned (anisotropic) or non-aligned extracellular matrix. Human fibroblasts were isolated from a patient tissues of vulval (VCAF8, VCAF2B) cervical (CerCAF), oral (OCAF), breast (1901T) carcinoma and metastatic melanoma (MAF2) and immortalised with lentiviral HTERT as described in Gaggioli, C., et al. Fibroblast derived matrix assays were performed as described in Franco-Barraza, J., et al and RNA extracted after 6 days in culture.