Generation of functional ciliated cholangiocytes from human pluripotent stem. Generation of functional ciliated cholangiocytes from human pluripotent stem

We report on the successful generation of functional ciliated cholangoocytes from hPSCs that will provide new opportunities to study and treat diseases of the liver. Both monolayer- and organoid-derived cells are adaptable for further bioengineering approcahes to produce more complex hPSC-deived liver tissue with biliary ductal structures. Additionally, the successful engraftment of differentiated cholangiocytes in the liver of immune-imcompetent mice provides the basis for developing novel therapeutic strategies to treat cholangiopathies. Overall design: Examination of heterogeneity and global transcriptome between 2D and 3D cholangiocytes