Information on collected samples, PCR primers and PCR performed in this study.

aReference [7] of main text.
bnumber of sequenced PCR reactions at ODU/UC respectively, “nd” entries indicate specific PCR reactions not performed on a given sample.
cClone sequences available by request to corresponding author.
dExtractions at ODU follow reference 18 and extractions at U of Copenhagen follow reference [14].
ePrimers used to generate 377 bp cytochrome b fragment from reference [11].
fThe 377 bp cytochrome b fragments were determined from extractions done in Munich, Germany (MediGenomix GmbH).
gThe 377 bp amplification did not yield rat sequence with this sample. Cytb.For2 was substituted for the original Forward primer and yielded rat sequences.
hAll samples were tested for the presence of trypanosomes. Only those that yielded trypanosome sequences are indicated.