Anaerobic microorganisms producing hydrogen and methane from the co-digestion of glycerol and domestic wastewater in single-stage and two-stage anaerobic fluidized bed reactors under mesophilic conditions. Metagenome

This study examines the co-digestion of crude glycerol with sanitary sewage in anaerobic fluidized bed reactors to evaluate their efficiency in hydrogen and methane production. Three anaerobic fluidized bed reactors were operated under mesophilic conditions: a control reactor functioning as a single-phase methanogenic reactor, and two reactors operating in sequence: an acidogenic reactor followed by a methanogenic reactor. The single-stage reactor was subjected to varying organic loading rates by altering hydraulic retention times and chemical oxygen demand concentrations. The acidogenic reactor was operated with a fixed hydraulic retention time of 2 hours and increasing matter organic concentrations, while methanogenic sequencing reactor received effluent from acidogenic reactor with varying hydraulic retention time.