Plasmids for MRG15 isoform expression and CDK1 promoter regulation
收藏科学数据银行2025-05-16 更新2026-04-23 收录
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https://www.scidb.cn/detail?dataSetId=d3c9cdc3057a453a99983a8258ed2a62
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资源简介：
This dataset includes a collection of plasmids constructed for functional studies of MRG15 splice variants and transcriptional regulation of CDK1. It comprises:1) Mammalian expression vectors for MRG15S and MRG15L isoforms, each fused with an N-terminal 3×FLAG tag and a C-terminal HA tag;2) Prokaryotic GST-fusion plasmids encoding full-length or N-terminal fragments of MRG15S and MRG15L, used for protein purification and pull-down assays in E. coli;3) A firefly luciferase reporter plasmid containing the full ~3.3 kb promoter region of CDK1 for promoter activity analysis in HEK293T cells.All plasmids were sequence-verified and are provided in GenBank (.gb), FASTA (.fasta), and SnapGene (.dna) formats.
提供机构：
Huayu Wang; Department of Biochemistry and Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China; MRG15 alternative splicing regulates CDK1 transcriptional activity in mouse cell senescence and myocardial regeneration
创建时间：
2025-05-16
