Cloning of Human ABCB11 Gene in E. coli required the removal of an Intragenic Pribnow-Schaller Box before it’s Insertion into Genomic Safe Harbor AAVS1 Site using CRISPR Cas9
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https://dataverse.harvard.edu/citation?persistentId=doi:10.7910/DVN/CJAODG
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资源简介：
Sequencing data and original images including some of the failed experiments can be found here.
Context: Please refer the manuscript.
Sequencing data:
1) Cloning of ABCB11--sequencing
2) Cloning of gRNA to two Cas9 vectors
3) Sequencing for ABCB11 integration to HEK293T cell line genome at AAVS1 site
4) Sequencing for off target sites-AAVS1 site HEK293T cell genomic DNA
PAGE and Western Blot Data:
1) E. Coli lysate transformed ad untransformed DH5a PAGE and W/B with anti ABCB11 antibody
2) ABCB11 transfected cell lines--whole protein fibroblasts
3) ABCB11 integration mediated by Cas9 cell line-whole protein fibroblasts
4) Puromycin selection cellines HEK
Agaraose gel images: T7 assay and PCRs
Comet Assay
提供机构：
Harvard Dataverse
创建时间：
2020-09-05
