Prevalence Rates of SFVcpz Infection in Wild Chimpanzees throughout Equatorial Africa.

aLocation of sites is shown in Figure 1.bP.t.v., P. t. verus; P.t.vl., P. t. vellerosus; P.t.t., P. t. troglodytes; P.t.s., P. t. schweinfurthii.cNumber of fecal samples tested for SFVcpz antibodies and/or viral RNA, with brackets indicating those tested by Western blot (WB) and those tested by RT-PCR, respectively.dNumber of fecal samples positive for SFVcpz antibodies and/or viral RNA, with brackets indicating those positive by WB and those positive by RT-PCR, respectively (the phylogenetic relationships of these newly derived SFVcpz strains are depicted in Figures 6�C8).eFor four habituated communities (TA, GM-MT, GM-KK, MH) the number of tested chimpanzees was known; for seven non-habituated communities (GT, DP, EK, BB, MB, LB, GM-KL) the number of tested chimpanzees was determined by microsatellite analysis of fecal DNA [33].fFor sites where the number of chimpanzees was known, SFVcpz prevalence rates (%, with brackets indicating 95% confidence intervals) were estimated based on the proportion of infected individuals, taking into account the ��field sensitivities�� of the antibody and virion RNA detection tests. For sites where the number of chimpanzees was not known, prevalence rates were estimated based on the number of fecal samples tested, assuming that a fraction (17%) was partially degraded and that any given chimpanzee was sampled on average 1.72 times (see Methods for details).gBased on mtDNA analysis, 24 samples were of P. t. vellerosus and 2 of P. t. troglodytes origin.h�C; not available.iFor this prevalence estimate, two WB indeterminant samples (reacting only with the Bet protein) were counted as negative.