Transcriptome analysis of the iron regulatory transcription factors in Cryptococcus neoformans in response to iron availability

Purpose: Understanding the iron-responsive regulatory networks in Cryptococcus neoformans. Methods: The transcriptome profiles of the wild-type, cir1 mutant and hapX mutant were generated by RNA-seq using Illumina Hiseq, in triplicate. The transcriptome profiles of each mutant was compared with that of the wild-type strain. Results: The iron-responsive transcription factors, Cir1 and HapX, are major regulators for iron acquisition and metabolism in C. neoformans. Overall design: Transcriptome of each mutant vs. Transcriptome of the wild-type