相关数据集
An Arrayed CRISPR Screen Identifies Knockout Combinations Improving Antibody Productivity in HEK293 Cells
Mammalian cells are used to express complex biologics, such as multispecific antibodies. While multispecifics enable promising new strategies for treating human disease, their production at high expre
NIAID Data Ecosystem30
Genome-scale CRISPRi Perturb-seq on both unstimulated and stimulated Jurkat cells reveals regulators of human T-cell signaling
The goal of the experiment is to identify genes involved in T-cell receptor (TCR) signaling and T-cell activation in human T cells. The Jurkat E6 cell line, engineered to express dCas9-KRAB and transd
NIAID Data Ecosystem20
Genes of CRISPR-Cas9 negative screening.
We compared changes in sgRNA abundance between T24 cells challenged and unchallenged by CD8+ T cells, and then performed negative screening (p < 0.01, log2Fold change < −1.0, and good sgRNAs ≥ 5). (X
NIAID Data Ecosystem10
Bcor loss perturbs myeloid differentiation and promotes leukaemogenesis [TargetedCRISPRscreen]
Investigating Bcor target genes in BcorΔE9-10KrasG12D leukaemia with a custom CRISPR dropout screen BcorΔE9-10KrasG12D cell line was transduced with a custom CRISPR library and a dropout screen was pe
NIAID Data Ecosystem30
Essential gene profiles for human pluripotent stem cells identify uncharacterized genes and substrate dependencies. Mair et al.
Human pluripotent stem cells (hPSCs) provide an invaluable tool for modeling diseases and hold promise for regenerative medicine. For understanding pluripotency and lineage differentiation mechanisms,
NIAID Data Ecosystem20
