microRNA sequencing in rat cardiac fibroblasts and fibroblast-derived exosomes

We found that cardiac fibroblasts produce and secrete exosomes. miRNA profiling and TaqMan qRT-PCR experiments identified miR-21 expression to be higher in cardiac fibroblasts compared to those of miR-21*, whereas in exosomes miR-21* expression was higher compared to miR-21. The purpose of the study was to validate these findings by miRNA sequencing in cardiac fibroblasts and fibroblasts-derived exosomes.本研究发现，心脏成纤维细胞可产生并分泌外泌体(exosomes)。通过miRNA表达谱分析(miRNA profiling)与TaqMan qRT-PCR实验，研究人员观测到：心脏成纤维细胞内miR-21的表达水平高于miR-21*；而在外泌体中，miR-21*的表达水平则显著高于miR-21。本研究旨在通过对心脏成纤维细胞及其衍生外泌体进行miRNA测序(miRNA sequencing)，验证上述实验结果。