Data underlying the publication: Sortase A-mediated farnesylation of Cdc42 in vitro

This dataset contains data collected for the publication <strong>Sortase A-mediated farnesylation of Cdc42 <em>in vitro.</em></strong>This dataset contains the following data:Figure 2a: SDS-PAGE, anti-His blot, anti-Cdc42 blotThe purpose of these SDS-PAGE and blots was to show indirect evidence of presence of the produced desired product Flag-Cdc42-farnesylFigure 3b: Silverstained SDS-PAGE and fluorescence image of SDS-PAGEThe purpose of these SDS-PAGEs was to visualize protein presence per fraction of the liposome co-floatation assay, an assay to show the membrane binding of Flag-Cdc42-farnesyl.Figure 3c: Band intensity data underlying the barplot, calculated barheights and standard error of the means, analysis script used for plottingThe purpose of these barplot was to visualized the quantification of protein presence per fraction of the liposome co-floatation assay, an assay to show the membrane binding of Flag-Cdc42-farnesyl.Figure 4b: Luminescence data underlying the barplot, calculated barheights and standard error of the means, analysis script used for plottingThe purpose of this assay was to show the GTPase activity of Flag-Cdc42-farnesyl and its enhancement of GTPase activity by GEF Cdc24.