Serial transmission of CJD-102L prions in transgenic mice homozygous for 102L or wild type human PrP.

IHC = immunohistochemistry; IB = immunoblotting; ND = not determined; Tg35c and Tg152c are congenic on FVB/N genetic background; sCJD = sporadic CJD; iCJD = iatrogenic CJD; DM, = dura mater; GH = growth hormone. * All inocula were first passaged in 102LL Tg27 transgenic mice to generate CJD-102L prions. † All samples were analysed by both IB and IHC using monoclonal antibodies ICSM 18 and ICSM 35. In 102LL Tg27 tissues ICSM 35 serves as a control to define non-specific background. # Positive either by clinical signs, immunoblotting and/or immunohistochemistry. ‡ All samples were positive for ICSM 18 but negative for ICSM 35. § Only 1 sample was available for analysis by IB. Serial transmission of CJD-102L prions in transgenic mice homozygous for 102L or wild type human PrP.